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pdquest 2 d analysis software (Bio-Rad)

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Bio-Rad pdquest 2 d analysis software

Tyrosine nitration pattern in potato leaves challenge-inoculated with Phytophthora infestans at 4 and 48 h post-inoculation (hpi) in the resistant genotype. Representative 2D electrophoresis (pH 4–7 for the first dimension) of potato-avr P. infestans stained with CBB G-250 and representative immunoblots probed with a polyclonal antibody against nitrotyrosine diluted at 1:1000. Molecular-mass standards (kDa) are indicated on the left. Arrowheads indicate all the immunoreactive spots, the symbols (s1–s45) refer to the proteins listed in <xref ref-type=

pdquest 2 d analysis software - by Bioz Stars, 2026-08

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1) Product Images from "Peroxynitrite-mediated tyrosine nitration modulates β-1,3-glucanase activity and potato defense against Phytophthora infestans"

Article Title: Peroxynitrite-mediated tyrosine nitration modulates β-1,3-glucanase activity and potato defense against Phytophthora infestans

Journal: Frontiers in Plant Science

doi: 10.3389/fpls.2026.1796014

Table 1 . The quantitative results for protein Tyr nitration were calculated using PDQuest 2-D Analysis Software (Bio-Rad), and the data were presented relative to the control sample, with the average set to 0 (control – mock-inoculated genotype). Asterisks indicate values that differ significantly from the control at *p < 0.05. " title="... results for protein Tyr nitration were calculated using PDQuest 2-D Analysis Software (Bio-Rad), and the data were ..." property="contentUrl" width="100%" height="100%"/>
Figure Legend Snippet: Tyrosine nitration pattern in potato leaves challenge-inoculated with Phytophthora infestans at 4 and 48 h post-inoculation (hpi) in the resistant genotype. Representative 2D electrophoresis (pH 4–7 for the first dimension) of potato-avr P. infestans stained with CBB G-250 and representative immunoblots probed with a polyclonal antibody against nitrotyrosine diluted at 1:1000. Molecular-mass standards (kDa) are indicated on the left. Arrowheads indicate all the immunoreactive spots, the symbols (s1–s45) refer to the proteins listed in Table 1 . The quantitative results for protein Tyr nitration were calculated using PDQuest 2-D Analysis Software (Bio-Rad), and the data were presented relative to the control sample, with the average set to 0 (control – mock-inoculated genotype). Asterisks indicate values that differ significantly from the control at *p < 0.05.

Techniques Used: Nitration, Two-Dimensional Gel Electrophoresis, Staining, Western Blot, Software, Control

Tyrosine nitration pattern in potato leaves challenge-inoculated with Phytophthora infestans at 4 and 48 h post-inoculation (hpi) in the susceptible genotype. Representative 2D electrophoresis (pH 4–7 for the first dimension) of potato-vr P. infestans stained with CBB G-250 and representative immunoblots probed with a polyclonal antibody against nitrotyrosine diluted at 1:1000. Molecular-mass standards (kDa) are indicated on the left. Arrowheads indicate all the immunoreactive spots, the symbols (s1–s32) refer to the proteins listed in <xref ref-type=

Table 1 . The quantitative results for protein Tyr nitration were calculated using PDQuest 2-D Analysis Software (Bio-Rad), and the data were presented relative to the control sample, with the average set to 0 (control – mock-inoculated genotype). Asterisks indicate values that differ significantly from the control at *p < 0.05. " title="... results for protein Tyr nitration were calculated using PDQuest 2-D Analysis Software (Bio-Rad), and the data were ..." property="contentUrl" width="100%" height="100%"/>
Figure Legend Snippet: Tyrosine nitration pattern in potato leaves challenge-inoculated with Phytophthora infestans at 4 and 48 h post-inoculation (hpi) in the susceptible genotype. Representative 2D electrophoresis (pH 4–7 for the first dimension) of potato-vr P. infestans stained with CBB G-250 and representative immunoblots probed with a polyclonal antibody against nitrotyrosine diluted at 1:1000. Molecular-mass standards (kDa) are indicated on the left. Arrowheads indicate all the immunoreactive spots, the symbols (s1–s32) refer to the proteins listed in Table 1 . The quantitative results for protein Tyr nitration were calculated using PDQuest 2-D Analysis Software (Bio-Rad), and the data were presented relative to the control sample, with the average set to 0 (control – mock-inoculated genotype). Asterisks indicate values that differ significantly from the control at *p < 0.05.

Techniques Used: Nitration, Two-Dimensional Gel Electrophoresis, Staining, Western Blot, Software, Control

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Journal: Frontiers in Plant Science

Article Title: Peroxynitrite-mediated tyrosine nitration modulates β-1,3-glucanase activity and potato defense against Phytophthora infestans

doi: 10.3389/fpls.2026.1796014

Figure Lengend Snippet: Tyrosine nitration pattern in potato leaves challenge-inoculated with Phytophthora infestans at 4 and 48 h post-inoculation (hpi) in the resistant genotype. Representative 2D electrophoresis (pH 4–7 for the first dimension) of potato-avr P. infestans stained with CBB G-250 and representative immunoblots probed with a polyclonal antibody against nitrotyrosine diluted at 1:1000. Molecular-mass standards (kDa) are indicated on the left. Arrowheads indicate all the immunoreactive spots, the symbols (s1–s45) refer to the proteins listed in Table 1 . The quantitative results for protein Tyr nitration were calculated using PDQuest 2-D Analysis Software (Bio-Rad), and the data were presented relative to the control sample, with the average set to 0 (control – mock-inoculated genotype). Asterisks indicate values that differ significantly from the control at *p < 0.05.

Article Snippet: Spot intensities were quantified using a ChemiDoc MP Imaging System (Bio-Rad) equipped with a high-resolution camera, and PDQuest 2-D Analysis Software (Bio-Rad).

Techniques: Nitration, Two-Dimensional Gel Electrophoresis, Staining, Western Blot, Software, Control

Journal: Frontiers in Plant Science

Article Title: Peroxynitrite-mediated tyrosine nitration modulates β-1,3-glucanase activity and potato defense against Phytophthora infestans

doi: 10.3389/fpls.2026.1796014

Figure Lengend Snippet: Tyrosine nitration pattern in potato leaves challenge-inoculated with Phytophthora infestans at 4 and 48 h post-inoculation (hpi) in the susceptible genotype. Representative 2D electrophoresis (pH 4–7 for the first dimension) of potato-vr P. infestans stained with CBB G-250 and representative immunoblots probed with a polyclonal antibody against nitrotyrosine diluted at 1:1000. Molecular-mass standards (kDa) are indicated on the left. Arrowheads indicate all the immunoreactive spots, the symbols (s1–s32) refer to the proteins listed in Table 1 . The quantitative results for protein Tyr nitration were calculated using PDQuest 2-D Analysis Software (Bio-Rad), and the data were presented relative to the control sample, with the average set to 0 (control – mock-inoculated genotype). Asterisks indicate values that differ significantly from the control at *p < 0.05.

Article Snippet: Spot intensities were quantified using a ChemiDoc MP Imaging System (Bio-Rad) equipped with a high-resolution camera, and PDQuest 2-D Analysis Software (Bio-Rad).

Techniques: Nitration, Two-Dimensional Gel Electrophoresis, Staining, Western Blot, Software, Control

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